Composite

Part:BBa_K101011:Design

Designed by: Kristen J Lindblad   Group: iGEM08_Minnesota   (2008-07-28)


Dual-Repressed Promoter for LacI and Lambda cI + RBS (med strength)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter contains two lambda cI operator sites; one upstream of the -35 polymerase binding site, and another between the -35 and -10 binding sites. It also includes a LacI operator site downstream of the -10 polymerase binding site. -35 and -10 sites were derived from the cI repressed promoter from lambda phage. A CAP binding site is also included for effective repression. For information concerning the RBS design, please see part BBa_B0032.


Source

This part combines BBa_K101001, the dually-repressed promoter, with BBa_B0032, a medium strength RBS with good characterization and experience by other registry users.

References