Composite
Part:BBa_K101011:Design
Designed by: Kristen J Lindblad Group: iGEM08_Minnesota (2008-07-28)
Dual-Repressed Promoter for LacI and Lambda cI + RBS (med strength)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This promoter contains two lambda cI operator sites; one upstream of the -35 polymerase binding site, and another between the -35 and -10 binding sites. It also includes a LacI operator site downstream of the -10 polymerase binding site. -35 and -10 sites were derived from the cI repressed promoter from lambda phage. A CAP binding site is also included for effective repression. For information concerning the RBS design, please see part BBa_B0032.
Source
This part combines BBa_K101001, the dually-repressed promoter, with BBa_B0032, a medium strength RBS with good characterization and experience by other registry users.